Characterization of transcriptomics during aging and genes required for lifespan in Drosophila intestine

作者：Yan Sang, Xiufan Ning, Qi Xu, Lan Wang, Yuhang Yan, Lijiao Zhang, Xiaolin Bi · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-98888-y · 被引用次数：7 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、Insect Utilization and Effects、Invertebrate Immune Response Mechanisms

Aging is closely associated with imbalanced transcription. Regulated transcription in different organs is significantly different during aging, indicating that organ-specific transcriptomics is critical for understanding this process. Here we analyze the transcriptomics of the intestines of 3-, 15-, 30-, 40- and 50-days old female flies, which include young, middle-aged, and old flies. We find that the differential expression of protein-coding genes and lncRNAs is significant in aging, and fly age is characterized by well-separated gene expression trajectories. The highly clustered differentially expressed genes are connected to specific biological processes and signalling pathways. In particular, the Imd and Toll pathways are the top two immune signalling pathways that are highly regulated, and members with increased expression in the Imd pathway span all upstream activating events and include many ubiquitylation-associated factors and regulators of NF-κB factor Relish. Increased expression of Toll pathway members includes sensing mediators for all kinds of microorganisms and multiple proteases in the proteolytic processing cascade. Moreover, the expression of molecular markers of intestinal cells is greatly changed. Enterocyte markers are the most significantly influenced, and enteroendocrine markers AstA and NPF, as well as intestinal stem cell (ISC)/enteroblast (EB) markers Esg and Klu are expressed at low levels in young flies and much higher levels in aged flies. Furthe...