Glutathione S-transferase in mediating adaptive responses of oats (Avena sativa) to osmotic and cadmium stress: genome-wide analysis

作者：Chenggang Xu, Lyu Jiang, Aixue Li, Jie Meng, Ping Yun, Jianfang Li, Changbin Liu, Yang Chen, Han Zhang, Hassan Ahmed Ibraheem Ahmed, Quan Q. Gao, Lana Shabala, Sergey Shabala, Bin Luo, Peichen Hou · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06559-x · 被引用次数：14 · 研究领域：Glutathione Transferases and Polymorphisms、Genomics, phytochemicals, and oxidative stress、Plant Stress Responses and Tolerance

Glutathione S-transferases (GSTs) are essential multifunctional enzymes. In the face of abiotic stresses such as drought and heavy metal exposure, plants utilize GSTs for detoxification and antioxidant defense, as these enzymes facilitate the conjugation of glutathione (GSH) with toxic compounds. Specific details of this process, however, remain unknown. This study identified 118 Avena sativa GST ( AsGST ) genes within the A. sativa genome and classified them into five subfamilies: Tau, Phi, Zeta, Lambda, and EF1Bγ. Phylogenetic analysis revealed that AsGSTs exhibit significant similarity to corresponding GST categories in Arabidopsis thaliana and Oryza sativa , indicating a possible common ancestor. Gene structure and conserved motif analysis demonstrated that AsGST genes within the same subfamily shares similarities in the number and positioning of exons and introns, as well as in motif composition, suggesting that these genes may perform analogous biological functions in A. sativa . The promoter regions of the identified genes are enriched with various cis-acting elements that play roles in plant growth and development, stress response, and hormone signaling. Transcriptomic analysis and real-time quantitative PCR (RT-qPCR) validation indicated that the expression of four AsGST genes ( AsGSTU12 , AsGSTU13 , AsGSTU14 , and AsGSTU15 ) was significantly up-regulated in the roots of A. sativa under both PEG-induced drought stress and CdCl 2 -induced cadmium stress. These genes ...