Lactobacillus paracasei fermented goji juice improves constipation in mice through inhibiting inflammation and regulating intestinal microecology

作者：Xiaoyu He, Yu Zhang, Haitian Fang, Ni Ma, Xueni Yan, Huabing Yang, Lu Cao, Lu Xu, Haiming Hu, Hongtao Liu · 发表于：Journal of Functional Foods · 年份：2025 · DOI：10.1016/j.jff.2025.106841 · 被引用次数：7 · 研究领域：Digestive system and related health、Diet and metabolism studies、Gastrointestinal motility and disorders

This study investigated the effects of Lactobacillus paracasei ( L. paracasei )-fermented Goji juice supernatant (FGS) on constipation. GC–MS data showed that the critical metabolites of FGS were nicotinamide, squalene, L-proline, melatonin, and sarcosine. FGS intervention significantly promoted intestinal peristalsis. Meanwhile, FGS ameliorated intestinal inflammation and mucosal barrier damage by inhibiting the expressions of pro-inflammatory factors (TNF-α, IL-1β, COX-2), promoting the expressions of Mucin 1, Mucin 3, ZO-1, AQP4, and ENac-γ. In addition, FGS treatment modulated gut microbiota composition, reduced the abundance of harmful bacteria ( Faecalibaculum , Bacteroides , Alloprevotella and Alistipes ), induced a bloom of probiotics, including Marvinbryantia , Eubacterium _ xylanophilum _group, Colidextribacter , and Candidatus _ Soleaferrea . Conclusively, this study demonstrated that FGS significantly reduced inflammatory response, prevented intestinal barrier damage, and modulated microbiota dysbiosis to improve constipation. Compared to unfermented Goji juice (UFGJ), FGS has shown a more effective anti-constipation effect, indicating that FGS could relieve constipation as a dietary therapeutic. • FGS ameliorated constipation by inhibiting the expressions of pro-inflammatory factors. • FGS improved mucosal barrier damage by up-regulated the transcription of Mucin 1, Mucin 3 and ZO-1. • FGS amended the intestinal microbiota and fecal metabolites in constipated mic...