Transcriptome and hormonal analyses reveal an important role of auxin and cytokinin in regulating rice ratooning ability

作者：Caihong Hu, Chenfan Zheng, Bo Liu, Like Sun, Jiaxi Yin, Yuanyuan Tan, Pedro García‐Caparrós, Qingyao Shu, Meng Jiang · 发表于：Plant Stress · 年份：2025 · DOI：10.1016/j.stress.2025.100866 · 被引用次数：4 · 研究领域：Rice Cultivation and Yield Improvement、Plant Genetic and Mutation Studies、Plant nutrient uptake and metabolism

• Ratooning ability (RA) in rice is influenced by both genetic and environmental factors. • With common genotypes of the known ratooning ability genes RRA3 and qRA2 , 23 rice varieties showed diverse RA phenotypes. • RNA-seq reveals key DEGs enriched in auxin and cytokinin pathways, with greater expression of cytokinin biosynthesis genes and lower expression of auxin biosynthesis genes in high RA varieties than in low RA variety. • High RA varieties have lower auxin and higher cytokinin levels, together with different expression of their biosynthesis genes, suggesting auxin and cytokinin play key roles in rice ratooning. Ratooning refers to the rapid regeneration of rice tillers from dormant buds located on the stubble following the harvest of the main crop. Rice ratoon cropping could significantly reduce labor and inputs demands, making it an attractive cultivation strategy in areas with suitable climatic conditions. A crucial determinant of rice yield is ratooning ability (RA), defined as the ratio of panicle number of the second to first crop. To date, only two genes associated with ratooning ability (RA), RRA3 and qRA2 , have already been identified; however, the genetic network and underlying biological mechanisms regulating RA remain largely unexplored. RRA3 has been shown to inhibit RA of rice by suppressing cytokinin signal transduction, while qRA2 promotes RA. In the present study, the RA trait was evaluated in 23 rice varieties cultivated across three different loca...