Optimal amino acid system for early embryo development in sows based on response surface methodology and high-throughput screening cell models

作者：Xinyu Wang, Jun Huang, Yanlong Li, Zhekun Zhu, Bangxin Xue, Yueyang Meng, Jiale Bao, Ning Ran, Siyu Li, Fang Chen, Shihai Zhang, Xiangzhou Zeng, Xiangzhou Zeng, Chuanjiang Cai, Chuanjiang Cai, Xiangfang Zeng, Xiangfang Zeng · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01194-w · 被引用次数：6 · 研究领域：Reproductive Biology and Fertility、Pluripotent Stem Cells Research、Birth, Development, and Health

BACKGROUND: Early embryo development plays a pivotal role in determining pregnancy outcomes, postnatal development, and lifelong health. Therefore, the strategic selection of functional nutrients to enhance embryo development is of paramount importance. In this study, we established a stable porcine trophectoderm cell line expressing dual fluorescent reporter genes driven by the CDX2 and TEAD4 gene promoter segments using lentiviral transfection. RESULTS: Three amino acid metabolites-kynurenic acid, taurine, and tryptamine-met the minimum z-score criteria of 2.0 for both luciferase and Renilla luciferase activities and were initially identified as potential metabolites for embryo development, with their beneficial effects validated by qPCR. Given that the identified metabolites are closely related to methionine, arginine, and tryptophan, we selected these three amino acids, using lysine as a standard, and employed response surface methodology combined with our high-throughput screening cell model to efficiently screen and optimize amino acid combination conducive to early embryo development. The optimized candidate amino acid system included lysine (1.87 mmol/L), methionine (0.82 mmol/L), tryptophan (0.23 mmol/L), and arginine (3 mmol/L), with the ratio of 1:0.43:0.12:1.60. In vitro experiments confirmed that this amino acid system enhances the expression of key genes involved in early embryonic development and improves in vitro embryo adhesion. Transcriptomic analysis of bla...