[Effect of electroacupuncture on the primary input level of dorsal root ganglion neurons under different stimuli observed by in vivo calcium imaging].

作者：Ninan Zhang, Xinyan Gao, Kun Liu, Q. Yu, Yang‐Shuai Su, Xiaoyu Wang, Zhiyun Zhang, Xianghong Jing · 发表于：PubMed · 年份：2025 · DOI：10.13702/j.1000-0607.20240065 · 被引用次数：1 · 研究领域：Neuroscience、Biology、Anatomy、Medicine、Pathology、Linguistics、Philosophy

OBJECTIVES: To observe the effect of electroacupuncture (EA) on the neuronal activity of primary afferent dorsal root ganglion (DRG) caused by different mechanical stimuli such as brush, clamp [clamping hind paw or "Zusanli" (ST36) acupoint] or stimulation at different temperatures (43 ℃, 51 ℃, 4 ℃), so as to explore the interaction between EA signals and different primary afferent signals at the DRG level. METHODS: The strength threshold of activated A fiber was determined by electrophysiological technique. DRG neurons were labeled by intrathecal injection of AAV9 virus, and the response of DRG neurons to different primary afferent stimuli was observed and recorded by calcium imaging. EA (0.5 mA, 0.2 ms, 2 Hz) was applied to "Zusanli" (ST36) on the right side for 10 min. The changes of the number, percentage of neurons of different sizes and fluorescence intensity of DRG neurons activated under different stimulation before and after EA were compared. RESULTS: <0.01), but had no significant effect on the percentage and fluorescence intensity of DRG neurons activated by 43 °C and 51 °C. CONCLUSIONS: EA at ST36 can inhibit the nociceptive mechanical input of the hind paw and the input of 4 °C cold stimulation.