Genetic mutations and insecticide resistance in Diaphorina citri : a comparative study across Chinese citrus regions

作者：Tianyuan Liu, Yu‐Xi Zhou, Chenyang Yuan, Yifan Gao, Jiayao Fan, Guo‐Rui Yuan, Jin‐Jun Wang, Wei Dou · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.8845 · 被引用次数：5 · 研究领域：Insect-Plant Interactions and Control、Phytoplasmas and Hemiptera pathogens、Insect and Pesticide Research

Abstract BACKGROUND Diaphorina citri , a serious pest of citrus, causes significant economic losses due to its role in spreading Huanglongbing. Chemical treatment is the primary method for controlling D. citri . In recent years, D. citri has demonstrated varying resistance levels to a number of pesticides. Combination bioassay and amplicon sequencing approaches can provide a more accurate assessment of resistance in D. citri . RESULTS Field populations of D. citri exhibited significant variability in pesticide susceptibility depending on geographic location. The Guilin population showed the highest imidacloprid sensitivity, with a median lethal concentration (LC 50 ) of 1.14 mg L −1 , indicating a comparatively high susceptibility to this pesticide. In contrast, the Chongzuo population exhibited the lowest sensitivity, with an LC 50 exceeding 2000 mg L −1 , reflecting a significant resistance level. Molecular analysis showed that resistance levels were associated with specific genetic mutations in two key areas: VGSC_L925M, VGSC_M918T, and the acetylcholine receptor α (nAChRα_N88S). Furthermore, there was a correlation between the rate of these mutations and the resistance level. Interestingly, the VGSC_L925M mutation was prevalent in the Chongzuo population, exhibiting a significant frequency of 42.08%. The nAChRα_N88S mutation, found at a low frequency of 0.24%, was unique to the Chongzuo population. CONCLUSION The frequency of VGSC_L925M mutations linked to pyrethroid resi...