Impact of fecal microbiota transplantation on lung function and gut microbiome in an ARDS rat model: A multi-omics analysis including 16S rRNA sequencing, metabolomics, and transcriptomics

作者：Dongwei Zhang, Zhenqiang Zhang, Longxiong Liao, Biying Dong, Xia Xiong, Xuejun Qin, Xianming Fan · 发表于：International Journal of Immunopathology and Pharmacology · 年份：2025 · DOI：10.1177/03946320251333982 · 被引用次数：7 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Barrier Structure and Function Studies

Objective: Acute respiratory distress syndrome (ARDS) is a severe pulmonary condition characterized by inflammation and lung damage, frequently resulting in poor clinical outcomes. Recent studies suggest that the gut-lung axis, mediated by gut microbiota, is critical in ARDS progression. This study investigates the therapeutic potential of fecal microbiota transplantation (FMT) in an ARDS rat model ( n = 6). Introduction: The pathogenesis of ARDS involves complex interactions between the lungs and gut, with microbiota playing a key role. Understanding the effects of FMT on lung function and gut microbiota may provide new therapeutic strategies for ARDS management. Methods: Sprague-Dawley rats were pre-treated with a broad-spectrum antibiotic cocktail to create a germ-free state and subsequently exposed to intranasal lipopolysaccharide to induce ARDS. The rats then received FMT treatment. Lung samples were analyzed using histopathology and transcriptomics. Fecal samples were analyzed using 16S rRNA sequencing and metabolomics. Results: FMT treatment significantly reduced lung injury and improved pulmonary function, as evidenced by increased partial pressure of arterial oxygen (PaO 2 ) and decreased partial pressure of arterial carbon dioxide (PaCO 2 ). FMT also significantly altered in gut microbiota composition by regulating the gut microbiota composition of Akkermansia and Lactobacillus , restoring the abundance of genera such as Muribaculaceae , Clostridia_UCG-014 , Prevote...