Effects of salinity on growth, survival, tissue structure, osmoregulation, metabolism, and antioxidant capacity of Eleutheronema tetradactylum (Shaw, 1804)

作者：Zongfa Chen, Weibin Liu, Anna Zheng, Minxuan Jin, Shunmei Huang, Lijuan Zhao, Shengliang Tang, Evodia Moses Mkulo, Linjuan Wang, Jun Zhang, Jianping Lu, Happiness Moses Mwemi, Baogui Tang, Hui Zhou, Bei Wang, Jiansheng Huang, Zhongliang Wang · 发表于：Frontiers in Marine Science · 年份：2025 · DOI：10.3389/fmars.2025.1553114 · 被引用次数：7 · 研究领域：Environmental Toxicology and Ecotoxicology、Physiological and biochemical adaptations、Aquaculture Nutrition and Growth

Introduction This study investigates the effects of salinity on the growth, survival, tissue morphology, osmotic regulation, metabolism, and antioxidant responses of juvenile Eleutheronema tetradactylum . Methods The experiment was conducted under controlled aquaculture conditions with eight salinity treatments (0, 5, 10, 15, 20, 25, 30, and 35 PSU), each with three replicates (20 fish per replicate) in cylindrical tanks (500 L). Juveniles (mean total length: 16.43 ± 0.87 cm; mean body weight: 35.71 ± 1.067g) were exposed to the treatments for 30 days. Key measurements included plasma osmotic pressure, ion concentrations, and Na+/K+-ATPase (NKA) in the gills, assessed at 0, 1, 10, 20, and 30 days. Results Survival rates, growth parameters, and histopathological changes in gill, intestinal, and kidney tissues were also evaluated. Additionally, plasma levels of lactic acid (LD), triglycerides (TG), glucose (GLU), total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) were measured. The results revealed that survival rates were significantly lower in the 0 PSU group compared to all other salinities ( P < 0.05). Growth performance, including specific growth rate (SGR), weight gain rate (WGR), and daily weight gain (DWG), was significantly reduced at high salinities (30 and 35 PSU) ( P < 0.05). Histopathological alterations were observed in the gills, intestine, and kidneys, particularly in osm...