Silkworm Cocoon—Derived Carbon Dots for Post-Trauma Hemostasis and Tissue Repair

作者：Xinru Wu, Miaomiao Yao, Xuan Qiao, Lintao Li, Zhiyun Meng, Shu‐Chen Liu, Yunbo Sun, Hui Gan, Xiaoxia Zhu, Zhuona Wu, Ruolan Gu, Guifang Dou · 发表于：Pharmaceuticals · 年份：2025 · DOI：10.3390/ph18050603 · 被引用次数：5 · 研究领域：Carbon and Quantum Dots Applications、Periodontal Regeneration and Treatments、Corneal Surgery and Treatments

Background: Traumatic hemorrhage management is challenging due to the need to control severe bleeding and support tissue repair. An ideal material would possess both hemostatic and wound-healing properties. Methods: Silkworm cocoon-derived carbon dots (SC-CDs) were synthesized via a hydrothermal method. After physical and chemical characterization using techniques such as HR-TEM and XPS, their hemostatic efficacy was assessed in rat liver injury, tail transection, and mouse coagulation disorder models. Moreover, the effects of the SC-CDs on platelet aggregation and activation were evaluated. The potential of the SC-CDs to promote wound healing was investigated through cell scratch assays and a mouse full-thickness skin defect model. Results: The SC-CDs showed a high quantum yield (12.9% ± 0.42%), with low hemolytic activity and cytotoxicity. In the hemostasis models, the SC-CDs significantly reduced the bleeding time and volume. In the rat liver injury model, the bleeding time was shortened from 152.67 ± 4.16 s (Control) to 55.33 ± 9.50 s (p < 0.05). In the rat tail transection model, the bleeding volume was reduced from 1.71 ± 0.16 g (Control) to 0.4 ± 0.11 g (p < 0.05). In the mouse coagulation disorder model, an 8 mg/kg dose reduced the bleeding volume to 11.80% ± 0.39% of that of the Control (p < 0.05). Mechanistic studies suggested enhanced platelet activation and aggregation. In the wound healing experiments, the SC-CDs reduced the wound area (88.53 ± 11.78 mm2...