Inhibition of SFRP1 by microRNA‑206‑3p may be the underlying cause of osteosarcopenia

作者：Yu Chen, Z. Lu, Yongjun Du, Yan Lv, Junhua Fang, Yu Zhao, Zhi Peng, Sheng Lu · 发表于：Biomedical Reports · 年份：2025 · DOI：10.3892/br.2025.1981 · 被引用次数：3 · 研究领域：RNA modifications and cancer、Bone health and treatments、RNA Research and Splicing

Osteosarcopenia is characterized by a simultaneous decrease in bone mass and muscle quality. Thus, determining the common pathogenesis between osteoporosis and sarcopenia may aid in identifying a solution. Secreted frizzled‑related protein 1 (SFRP1), a Wnt/β‑catenin pathway inhibitor, reportedly decreases during the osteogenesis process and is increased in osteoporosis and sarcopenia mice models. As microRNAs (miRNAs/miRs) can regulate the expression of multiple proteins, the present study aimed to determine if miR‑206‑3p can promote the nuclear translocation of β‑catenin by inhibiting SFRP1 during both osteogenesis and myogenesis. Transcriptome sequencing revealed that SFRP1 was markedly upregulated in the BMSCs derived from ovariectomized mice. In vitro induction of osteogenesis confirmed that SFRP1 negatively regulated osteogenesis. A luciferase reporter assay confirmed that miR‑206‑3p downregulated SFRP1 by directly binding to the 3' untranslated region. Subsequently, the BMSC and L6 cells were transfected with an miR‑206‑3p inhibitor or a corresponding negative control. Immunoblotting was performed to assess the relative expression levels of SFRP1 and Wnt/β‑catenin signaling. The mRNA levels of SFRP1, osteogenesis‑related molecules and myogenesis‑related molecules were also detected by quantitative real‑time PCR. The miR‑206‑3p inhibitor reduced the expression of osteogenesis‑ and myogenesis‑related molecules and inactivated the Wnt/β‑catenin signal...