Evaluation of natural l ‐phenylalanine‐derived Amidohydrazide derivatives in ensuring agriculture production against phytopathogenic fungi †

作者：Tiancheng Zhang, Hong‐Bin Fang, Yu‐Fei Gong, Wenfei Zhang, Jia‐Cheng Liu, Jing Chang, Zhan‐Fang Chen, Lufei Zhao, Yu‐Cheng Gu, Xuewen Hua · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.8856 · 被引用次数：8 · 研究领域：Fungal Plant Pathogen Control、Plant Disease Resistance and Genetics、Microbial Natural Products and Biosynthesis

Abstract BACKGROUND Ensuring food security is fundamental to national security and an important guarantee for global peace and development. The use of fungicides represents an effective strategy in safeguarding sufficient food supply against phytopathogenic fungi; however, given the escalating pathogen resistance, there is an urgent need to develop new, highly efficient, and environmentally friendly green fungicides. RESULTS In this study, 24 novel amidohydrazide derivatives were designed and synthesized by incorporating the bioactive amide and hydrazide groups into natural l ‐phenylalanine. The bioassays revealed that certain compounds exhibited remarkable inhibitory activity against agricultural pathogenic fungi, with compound A21 particularly displaying exceptional in vitro and in vivo fungicidal activity against Rhizoctonia solani . Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations showed that treatment with compound A21 induced significant morphological changes in R. solani mycelia, including the cell membrane contraction. Transcriptomic analysis indicated that differentially expressed genes (DEGs) in R. solani treated with compound A21 were significantly enriched in pathways related to the carbohydrate metabolism and lipid metabolism, and the relative expression of representative DEGs was further validated by quantitative real‐time polymerase chain reaction (qRT‐PCR). The increase in ergosterol content suggested that R. solani ma...