Quantitation of common mycotoxins (aflatoxin B1 and deoxynivalenol) in cereals by high performance thin layer chromatography with smartphone image capture and ImageJ analysis

作者：Xudong Shi, Xingjun Xi, Xiaoqian Tang, Yisheng Chen · 发表于：Journal of Future Foods · 年份：2025 · DOI：10.1016/j.jfutfo.2024.08.011 · 被引用次数：12 · 研究领域：Mycotoxins in Agriculture and Food、Wheat and Barley Genetics and Pathology、Plant Pathogens and Resistance

• A method based on HPTLC-image quantification analysis was established for the cost-effective and rapid preliminary screening of AFB1 and DON in grain samples. • The HPTLC-ImageJ analysis method enables the specific identification and quantitative analysis of AFB1 and vomitoxin in grain samples. • The combination of ImageJ, smartphone image capture, and HPTLC offers a cost-effective method for analysis. • Quantitative analysis using ImageJ is recognized as an ideal choice for laboratories operating in resource-limited settings, as it provides reliable image analysis results under cost constraints. Aflatoxin B 1 (AFB 1 ) and deoxynivalenol (DON) are among the most frequently detected mycotoxin in cereals, posing great challenge to human health. This work proposed a cost-efficient and robust planar chromatography method for the rapid screening AFB 1 in millet and buckwheat, as well as DON in wheat, corn, barley, and oats, achieving detection of the two common fungal toxins in cereals. First, the sample preparation was realized by a simple extraction with 10 mL of acetonitrile, both containing 4 g of anhydrous MgSO 4 , 1 g of NaCl, and 25 mg of primary secondary amine adsorbent. After high throughput chromatography, which resulted in the separation of the targeted compound from background noise and derivatization with 10% ethanol solution of AlCl 3 (only for the visualization of DON). The separation results were recorded using a smartphone in a dark chamber equipped with a 366 ...