STM is required for fate establishment of productive shoot progenitor cells in Arabidopsis tissue culture

作者：Ning Zhai, Dixiang Xie, Lin Xu · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2025 · DOI：10.1101/2025.04.14.648630 · 研究领域：Plant Molecular Biology Research、Plant tissue culture and regeneration、Plant Reproductive Biology

Abstract De novo shoot regeneration via the two-step tissue culture method is widely used for vegetative reproduction of plants and for gene transformation and editing. A key step in tissue culture is the fate transition from shoot progenitor cells (SPCs) to productive SPCs (prSPCs), which are capable of differentiating into a shoot. In this study, we identified the class I KNOTTED1-like homeobox (KNOX) transcription factor gene SHOOT-MERISTEMLESS ( STM ) as the key controller of, and marker gene for, the fate transition from SPCs into prSPCs, indicating that STM might serve as a candidate of molecular tools to improve de novo shoot regeneration in the agricultural applications.