Genome-wide identification of pyrabactin resistance 1-like (PYL) gene family under phytohormones and drought stresses in alfalfa (Medicago sativa)

作者：Kun Wang, Jiao Cheng, Jingru Chen, Yan-Yan Luo, Yuheng Yao, Lili Nan · 发表于：BMC Genomics · 年份：2025 · DOI：10.1186/s12864-025-11575-0 · 被引用次数：4 · 研究领域：Plant Stress Responses and Tolerance、Calcium signaling and nucleotide metabolism、Plant-Microbe Interactions and Immunity

BACKGROUND: The Pyrabactin resistance 1-like proteins (PYR/PYL/RCAR) protein plays a critical regulatory role in the ABA signal transduction pathway as a direct receptor of abscisic acid (ABA). Although PYL genes have been identified in a variety of plants, the evolution and structural characteristics of these genes in alfalfa (Medicago sativa) are largely unknown. Therefore, a comprehensive bioinformatics analysis of the PYL gene family was performed in this research. RESULTS: The results indicated that 41 MsPYL genes were unevenly distributed across 24 chromosomes. According to gene structure, conservative features, and phylogenetic relationships, MsPYL proteins can be divided into 6 groups, all of which have PYR/PYL/RCAR domains, and MsPYL proteins are relatively small (molecular weight 19.59 kDa to 25.31 kDa). MsPYL genes contains cis-acting elements that has functions in plant growth and development, hormone regulation, and stress response. Furthermore, transcriptome data showed that drought stress affected the MsPYL genes' expression levels in alfalfa. Tissue specificity analysis revealed that all MsPYL genes exhibited varying responses to drought, abscisic acid (ABA), salicylic acid (SA), and indole-3-acetic acid (IAA). Additionally, all MsPYL genes were expressed to different extents in both the aboveground and underground tissues following stimulation. They were induced by IAA, ABA, and SA from 6 h to 12 h, and ABA induced MsPYL4 most significantly at the 12 h mark. ...