Extreme temperatures modulate gene expression in the airway epithelium of the lungs in mice and asthma patients

作者：Firdian Makrufardi, Syue-Wei Peng, Kian Fan Chung, Marc Chadeau‐Hyam, Kang-Yun Lee, Ta-Chih Hsiao, Kin‐Fai Ho, Desy Rusmawatiningtyas, Indah Kartika Murni, Eggi Arguni, Yuan‐Hung Wang, Shu‐Chuan Ho, Feng‐Ming Yang, Kai-Jen Chuang, Sheng-Chieh Lin, Hsiao‐Chi Chuang · 发表于：Frontiers in Medicine · 年份：2025 · DOI：10.3389/fmed.2025.1531154 · 被引用次数：3 · 研究领域：Thermal Regulation in Medicine、Climate Change and Health Impacts、Heat shock proteins research

Background: The objective of this study was to examine the effects of extreme temperatures on the gene signature and pathways of airway epithelial cells in mice and asthma patients. Methods: -D mice and pediatric and adult patient's airway epithelial exposed to extreme temperatures. Results: We observed that Mmp8, Sftpb, Cxcl15 and Cd14 were significantly upregulated in airway epithelial cells in mice model. Cma1, Kit, Fdx1, Elf1a, Cdkn2aipnl, Htatsf1, Mfsd13a, Gtf2h5, Tiam2, and Trmt10c were significantly upregulated in 40°C exposure in airway epithelial cells. Sftpc, Gpr171, Sic34a2, Cox14, Lamp3, Luc7l, Nxnl, Tmub2, Tob1, and Cd3e genes were significantly upregulated in 10°C exposure group. Pediatric asthma subjects in the extreme high temperature group consistently showed decreased Wfdc21, Cib3, and Sftpc, at the same time increased Tiam2 and Cma1 expression, while in the extreme low temperature group exhibited consistently higher expression of Sftpc and Nxnl, at the same time decreased Wfdc21, Cib3, Cma1, and Dld expression. Notably, the mice in the extreme temperature fluctuation group showed decreased Wfdc21, Cib3, Gpr171, and Cttnbp2 expression, while increased Hbb-bs expression. Adult asthma subjects in the extreme temperature fluctuation group showed consistently decreased Wfdc21, Cib3, Gpr171, and Cttnbp2 expression, while increased Tiam2 and Cma1 expression. We observed that the mild, moderate, and severe asthma subject in the extreme low temperature group showed ...