The synergistic effects of humic acid, chitosan and Bacillus subtilis on tomato growth and against plant diseases

作者：Cunpu Qiu, Ying Bao, Dingding Lü, Mengyuan Yan, Guilong Li, Kai Liu, Shiping Wei, Meng Wu, Zhongpei Li · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1574765 · 被引用次数：18 · 研究领域：Plant Growth Enhancement Techniques、Plant-Microbe Interactions and Immunity、Composting and Vermicomposting Techniques

Understanding the effects of bio-antimicrobial materials on plant growth and against diseases and the relevant mechanisms are highly important for sustainable soil use and plant safety production. This study explored the impacts and corresponding mechanisms of the combined utilization of humic acid, chitosan, and Bacillus subtilis ( B. subtilis ) on tomato growth and diseases occurrence through a greenhouse pot experiment. The plant height, fresh weight, disease index, rhizosphere microbial community, and root exudates composition of tomatoes were determined. With the combined application of humic acid, chitosan and B. subtilis (HBC), the height and fresh weight of tomato plants were significantly increased ( p < 0.05), and the incidence of plant diseases was decreased by 45.1%. In HBC treatment, the diversity of fungal and bacterial communities was notably enhanced. The relative abundances of Bacillus, Gemmatimonas, Neobacillus, Acinetobacter, Humicola increased, while the relative abundances of Sphingomonas, especially soil-borne plant pathogen Fusarium and Ralstonia, significantly decreased ( p < 0.05). Besides the increased diversity of root exudates, the content of phenolic acids, which are allelochemicals related to continuous cropping disorder, decreased. The results of cooccurrence network analysis indicated that the abundances of Eicosanoids, Fatty acids and conjugates, and Flavonoid lycosides compounds in root exudates, which are positively correlated ...