Metabolic reprogramming by chemo-gene co-delivery nanoparticles for chemo-immunotherapy in head and neck squamous cell carcinoma

作者：Wenqing Zou, Bingyue Huo, Yaqin Tu, Yuhe Zhu, Yuwei Hu, Qianru Li, Xuan Yu, Bo Liu, Wei Tang, Songwei Tan, Hongjun Xiao · 发表于：Acta Biomaterialia · 年份：2025 · DOI：10.1016/j.actbio.2025.04.031 · 被引用次数：22 · 研究领域：Graphene and Nanomaterials Applications、Nanoparticle-Based Drug Delivery、Cancer Research and Treatments

The therapeutic effects of platinum-based drugs are closely linked to the dysregulation of tumor metabolic-immune microenvironment, particularly aberrant lactate accumulation. Herein, we engineered multifunctional nanoparticles (PPPt IV NPs) through electrostatic self-assembly of poly(β-amino ester) to co-encapsulate a cisplatin prodrug (Pt IV ) and CRISPR/Cas9-PKM2 plasmids. Mechanistically, PPPt IV NPs efficiently entered cells via endocytosis , followed by escape from lysosomal degradation and cargo release. The reduction of Pt IV prodrug to active Pt II via GSH depletion induced DNA damage and ROS upregulation, thereby triggering apoptosis. Concurrently, CRISPR/Cas9-mediated PKM2 knockdown suppressed the Warburg effect , resulting in reduced lactate production and downregulated expression of HIF-1α and PD-L1. These alterations drove immune microenvironment remodeling through enhanced dendritic cell maturation, polarized M1 macrophages, and altered cytokine profiles (characterized by upregulation of IFN-γ, TNF-α, and IL-12 alongside suppression of IL-10), ultimately activating T cell-mediated antitumor immunity. Compared to conventional cisplatin , PPPt IV NPs demonstrated superior efficacy against both primary and recurrent tumors while reducing nephrotoxicity through synergistic chemo-immunotherapeutic effects, offering a valuable strategy for HNSCC treatment. Statement of significance This study engineered an innovative nanoplatform (PPPt IV ) that synergistically integ...