Modular Engineering of a Synthetic Biology-Based Platform for Sustainable Bioremediation of Residual Antibiotics in Aquatic Environments

作者：Hao Ren, Meilin Qin, Lin Zhang, Zhen Li, Yuze Li, He Qian, Jiahao Zhong, Donghao Zhao, Xin‐Lei Lian, Hongxia Jiang, Xiao‐Ping Liao, Jian Sun · 发表于：Engineering · 年份：2025 · DOI：10.1016/j.eng.2025.03.033 · 被引用次数：8 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Biosensors and Analytical Detection

• FerTiG is designed as a multienzyme complex to degrade tetracycline residues; • GDH module fuels FerTiG to spontaneously degrade tetracycline residues; • Ferritin module renders FerTiG resilient to the environmental stresses; • FerTiG robustly eliminated tetracycline from multiple environment matrices; • FerTiG displays no eco- or biosafety concerns for application. Tetracycline (TC) residues from anthropogenic activities undesirably present in nature as an emerging sustainability challenge and thereby require innovations in remediation technologies. Herein, as inspired by the microcompartment structure in living organisms, we adopt a synthetic biology approach to engineer the FerTiG, a modular enzyme assembly, to robustly scavenge TC residues with improved performance. The FerTiG consists of three functional modules, namely, a TC degradation module (Tet(X4)), a cofactor recycling module glucose dehydrogenase (GDH) , and a protection module (ferritin), to organize diverse catalytic processes simultaneously as a biological circuit. The incorporation of GDH suitably fuels the FerTiG-dependent TC degradation by regenerating expensive nicotinamide adenine dinucleotide phosphate (NADPH) cofactor with glucose. The ferritin shields the catalytic core of FerTiG to resiliently decompose TC under unfavorable conditions. Due to collaboration among functional modules, FerTiG strongly catalyzes the residual TC removal from multiple environmental matrices. The degradation pathways and en...