Total Synthesis of Auriculatin and Millexatin F and Discovery of Their Antibacterial Mechanism

作者：Yufei Che, Qiying Wang, Zhanjun Hou, Jun Xie, Ling Mei, Ting Peng, Chunran Zhang, Tao Song, Hongbo Dong · 发表于：ACS Omega · 年份：2025 · DOI：10.1021/acsomega.4c10340 · 被引用次数：8 · 研究领域：Microbial Natural Products and Biosynthesis、Marine Sponges and Natural Products、Bioactive Compounds and Antitumor Agents

High Resolution Image Download MS PowerPoint Slide The emergence of multidrug-resistant (MDR) bacteria necessitates the urgent development of novel antibacterial agents. This study reported the first total synthesis of two antibacterial isoflavones, auriculatin ( 1 ) and millexatin F ( 2 ), derived from the tropical medicinal plant Millettia extensa . Through in vitro evaluations, both compounds 1 and 2 possessed significant antibacterial activities (MICs = 0.5–4 μg/mL) and rapid bactericidal properties against Gram-positive bacteria, along with high safety for mammalian cells. Mechanistic studies revealed that auriculatin ( 1 ) and millexatin F ( 2 ) interact with bacterial cell membranes, inducing alterations in bacterial morphology and membrane permeability and inducing a rise in the leakage of intracellular DNA and proteins, thereby leading to bacterial death. In addition, our studies indicated that millexatin F ( 2 ) could interact with phosphatidylethanolamine (PE) and cardiolipin (CL) of cytoplasmic membranes in both Gram-positive and Gram-negative bacteria. Furthermore, millexatin F ( 2 ) showed increased efficacy against Gram-negative bacteria when combined with a permeabilizer (polymyxin B nonapeptide), indicating potential for broader application. These findings underscore the therapeutic promise of auriculatin ( 1 ) and millexatin F ( 2 ) as lead candidates in the fight against bacterial infections.