Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44–AKT–CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation

作者：Xi Jin, Zhongyu Jian, Yucheng Ma, Jun Wen, Ningning Chao, Xiaoting Chen, Liyuan Xiang, Yiqiong Yuan, Linhu Liu, Ya Li, Jingwen Wei, Banghua Liao, Li Zhang, Kunjie Wang · 发表于：Research · 年份：2025 · DOI：10.34133/research.0690 · 被引用次数：9 · 研究领域：Kidney Stones and Urolithiasis Treatments

Oxalate-induced crystalline kidney injury is a common form of crystal nephropathy. The accumulation of calcium oxalate (CaOx) crystal could lead to renal epithelium injury and inflammation. The underlying cellular events in kidney after CaOx crystal formation are largely unknown. This study was aimed to gain a better understanding of mouse kidney function in the development of renal CaOx formation. The study utilized a mouse CaOx model to analyze the cellular response at 5 time points using single-cell RNA sequencing and investigate the interaction of different cells during renal CaOx crystal formation. Additionally, the study investigated the communication between these cells and macrophages, as well as the role of chemokines in recruiting infiltrating macrophages. RNA velocity analysis uncovered an alternative differentiation pathway for injured and S1 proximal tubule cells, which mainly communicate with macrophages through the SPP1-CD44 pair, along with the expression of proinflammatory factors and stone matrix genes during renal CaOx crystal formation. Furthermore, resident Fn1 macrophages were found to express chemokines, such as CCL2, which recruited infiltrating macrophages. The CCL2 secretion was mediated by the CD44-AKT pathway. Blocking CCL2 decreased the expression of injury markers in the kidney, including CLU, LCN2, and KIM-1, and inhibited CaOx crystal deposition. The study identified potential cell types and target genes involved in renal tubule injury in oxala...