Structural characterization and bioactivity evaluation of a soluble polysaccharide (PCHP-1A) from Polygonatum cyrtonema Hua: antioxidant and α-glucosidase inhibitory activities

作者：Lunbin Xia, Cunwu Chen, Shao-Shuai Bi, Yafei Zhang, Nai‐Dong Chen, Jing Feng · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00768-0 · 被引用次数：7 · 研究领域：Polysaccharides and Plant Cell Walls、Polysaccharides Composition and Applications、Microbial Metabolites in Food Biotechnology

In recent years, the biological activities of polysaccharides derived from Chinese herbal medicones have garnered increasing attention. Polygonatum cyrtonema Hua, a widely used traditional Chinese medicinal herb, possesses significant healthcare value. However, the structure–activity relationship between polysaccharide structures and biological functions remains to be fully elucidated. Crude Polygonatum cyrtonema Hua polysaccharides (PCHP) were extracted using hot water extraction followed by alcohol precipitation. The extract was subsequently purified through DEAE seplife FF chromatography, yielding two franctions: PCHP-1 and PCHP-2. Because of the highest content and the better solubility, PCHP-1 was further purified using Sephacryl S-400HR column chromatography to obtain a purified fraction designated as PCHP-1A. Subsequently, The structural characteristics of PCHP-1A were comprehensively analyzed through monosaccharide composition analysis, scanning electron microscopy (SEM), Fourier transform-infrared spectroscopy (FT-IR), methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy. Additionally, the antioxidant and hypoglycemic activities of PCHP-1A were evaluated though in vitro experiments. Structural analysis revealed that PCHP-1A, with an average molecular weight of 2.90 × 104 Da, is comprised of fructose (Fru), glucose (Glc), galactose (Gal), mannose (Man), arabinose (Ara) and rhamnose (Rha). PCHP-1A exhibited a compact and curly spherical molecule conf...