Serum exosomal hsa-miR-142–5p, hsa-miR-1908–5p, and hsa-miR-450b–5p as candidate biomarkers for recurrent depressive disorder diagnosis and ECT treatment response: A preliminary investigation

作者：Meng Liu, Ke Fang, Xiaorui Wang, Kun Wang, Lihong Zhang, Mingliang He, Yanyan Xu, Yuan Wu, Jin‐Fang Ge · 发表于：Brain Research Bulletin · 年份：2025 · DOI：10.1016/j.brainresbull.2025.111345 · 被引用次数：4 · 研究领域：Electroconvulsive Therapy Studies、Extracellular vesicles in disease、Phosphodiesterase function and regulation

PURPOSE: This study investigated the differential expression of serum exosomal miRNAs in female patients with recurrent depressive disorder (RDD) before and after non-convulsive electroconvulsive therapy (ECT), aiming to explore potential diagnostic and therapeutic biomarkers. METHOD: Serum samples were collected from three groups: healthy female volunteers aged 30-50, female patients with RDD prior to ECT, and female patients post-ECT who had achieved remission. Exosomes were isolated from serum, identified through transmission electron microscopy, nanoparticle tracking analysis, and Western blot analysis of exosomal markers. Total RNA was extracted from exosomes, and miRNA sequencing was conducted to identify differentially expressed miRNAs. Gene target prediction, Gene Ontology, and KEGG pathway enrichment analyses were also performed. RESULTS: miRNA sequencing revealed significant differences in exosomal miRNA profiles among the three groups. Compared to controls, 69 miRNAs were upregulated and 98 downregulated in the model group, while the recovery group showed 41 upregulated and 51 downregulated miRNAs compared to the model group. Furthermore, the recovery group exhibited 35 upregulated and 59 downregulated miRNAs compared to controls. Analysis identified hsa-miR-142-5p, hsa-miR-1908-5p, and hsa-miR-450b-5p as potential biomarkers for RDD diagnosis and ECT treatment response, with functional roles likely related to inflammation, neurotransmission, and synaptic plasticit...