Multiplex Detection of Cd 2+ , Hg 2+ , Glutathione, and Ascorbic Acid Using NaYF 4 :Yb,Er@NaYF 4 Core–Shell Upconversion Nanoparticles and Trithiocyanuric Acid-Modified Gold Nanoparticles

作者：Hanqing Liu, Yifei Zhou, Jing Xu, Le Ding, Chuan Liao, Tao Wang, Haifeng Zhou, Guangjun Zhou · 发表于：ACS Applied Nano Materials · 年份：2025 · DOI：10.1021/acsanm.5c00399 · 被引用次数：5 · 研究领域：Analytical chemistry methods development、Luminescence Properties of Advanced Materials、Molecular Sensors and Ion Detection

High-efficiency detection of cadmium ions (Cd 2+ ) and mercury ions (Hg 2+ ) is crucial due to their harmful effects on the environment and human health. Here, we developed a ratiometric upconversion fluorescence (UCF)-based nanosensor by incorporating lanthanide-doped upconversion nanoparticles (UCNPs) and gold nanoparticles modified with trithiocyanuric acid (TMT-AuNPs) as specific recognition elements for the highly sensitive and selective real-time detection of Cd 2+ and Hg 2+ . The developed nanosensor exhibited selective absorbance enhancement and UCF quenching behavior with the addition of Cd 2+ and Hg 2+ based on the inner filter effect (IFE). The sensitivity of the nanosensor was significantly enhanced by converting the change in absorbance into an exponential variation in UCF intensity. Therefore, the quantitative detection of Cd 2+ and Hg 2+ was achieved by measuring the UCF ratio I 654 / I 540 . In order to distinguish between Cd 2+ and Hg 2+, two biomolecules, glutathione (GSH) and ascorbic acid (AA), were used based on their different interactions with Cd 2+ and Hg 2+ . GSH formed a specific tetrahedral complex (GSH) 4 Cd with Cd 2+, thereby preventing Cd 2+ from inducing changes in UCF intensity. In contrast, AA specifically reduced Hg 2+, thereby preventing Hg 2+ -induced UCF changes. Thus, GSH and AA could also be detected. The limits of detection (LODs) of Cd 2+, Hg 2+, GSH, and AA were 2.8, 5.2, 5.1, and 7.2 nM, respectively. The designed detection platform...