PITPβ promotes COPI vesicle fission through lipid transfer and membrane contact formation

作者：Kunyou Park, Sungeun Ju, Hyewon Choi, Peng Gao, Geul Bang, Jung Hoon Choi, Jiwon Jang, Andrew J. Morris, Byung‐Ho Kang, Victor W. Hsu, Seung‐Yeol Park · 发表于：The Journal of Cell Biology · 年份：2025 · DOI：10.1083/jcb.202407166 · 被引用次数：2 · 研究领域：Cellular transport and secretion、Lipid Membrane Structure and Behavior、Endoplasmic Reticulum Stress and Disease

Intracellular transport among organellar compartments occurs in two general ways: by membrane-bound carriers and membrane contacts. Specific circumstances that involve the coordination of these two modes of transport remain to be defined. By studying coat protein I (COPI) transport, we find that phosphatidylcholine with short acyl chains (sPC) is delivered through membrane contact from the endoplasmic reticulum (ER) to sites of COPI vesicle formation at the Golgi to support the fission stage. Phosphatidylinositol transfer protein beta (PITPβ) plays a key role in this process, with the elucidation of this role shedding new insights into how PITPβ acts, providing a mechanistic understanding of a specific circumstance when vesicular transport requires membrane contact and contributing to the general understanding of how intracellular transport carriers are formed.