Distinct composition and metabolic potential of biliary microbiota in patients with malignant bile duct obstruction

作者：Lijuan Qian, Limei Wang, Zigui Zou, Fujuan Luan, Xiaodi Cai, Jikai Zhou, Dongming Zhu, Jianxin Ye, Chenguang Dai · 发表于：European Journal of Gastroenterology & Hepatology · 年份：2025 · DOI：10.1097/meg.0000000000002948 · 被引用次数：6 · 研究领域：Gut microbiota and health、Gallbladder and Bile Duct Disorders、Cholangiocarcinoma and Gallbladder Cancer Studies

BACKGROUND: Emerging evidence highlights the role of biliary microbiota in hepato-biliary-pancreatic diseases. The characteristics of biliary microbiota in malignant bile duct obstruction remain poorly understood. This study aims to investigate the composition and metabolic functions of biliary microbiota in patients with malignant obstruction. METHODS: Eligible patients were enrolled in this prospective study at First Affiliated Hospital of Soochow University between December 2022 and October 2023, including distal cholangiocarcinoma, hilar cholangiocarcinoma, pancreatic ductal adenocarcinoma, periampullary carcinoma, and gallbladder carcinoma. The patients with choledocholithiasis served as controls. Bile samples were collected via endoscopic retrograde cholangiopancreatography. Microbiota identification was performed using 16S rRNA sequencing, while bile acids were analyzed using mass spectrometry. RESULTS: A total of 56 patients were successfully enrolled in this study, 25 in the tumor group and 31 in the stone group. A distinct biliary microbial community was observed in patients with malignant bile duct obstruction, consisting of Prevotella, Uruburuella, Atopostipes, Clostridium IV, Halomonas, Tannerella, Porphyromonas, Achromobacter, Rouxiella, Campylobacter, Corynebacterium, Turicibacter, Muribaculum, Selenomonas, and Alloprevotella at genus level. Notably, Clostridium IV, Halomonas, Rouxiella, and Turicibacter were exclusively present in the tumor group. Bile acid le...