Chronic Arsenic Exposure Causes Alzheimer's Disease Characteristic Effects and the Intervention of Fecal Microbiota Transplantation in Rats

作者：Shuyuan Li, Jia Li, Kun Chen, Jing Wang, Longmei Wang, Chao Feng, Kanglin Wang, Yifan Xu, Yi Gao, Xiaoyan Yan, Qian Zhao, Ben Li, Yulan Qiu · 发表于：Journal of Applied Toxicology · 年份：2025 · DOI：10.1002/jat.4782 · 被引用次数：4 · 研究领域：Gut microbiota and health、Arsenic contamination and mitigation、Heavy Metal Exposure and Toxicity

ABSTRACT Arsenic exposure and intestinal microbiota disorders may be related with Alzheimer's disease ( AD ), but the mechanism has not been elucidated. This study conducted chronic arsenic exposure from rat's maternal body to adult offspring to investigate the mechanisms of the characteristic effects of chronic arsenic exposure on AD, and further explored the intervention effect of fecal microbiota transplantation (FMT) on arsenic‐mediated neurotoxicity. Transmission electron microscopy, HE staining, and related indicators were measured in the control group, the exposed group, and the FMT intervention group. Western blot was used to determine microtubule‐associated proteins Tau and p‐Tau 396 , intestinal–brain barrier–related proteins Claudin‐1 and Occludin, ELISA was used to detect the content of Aβ 1–42 , and 16S rRNA sequencing was used to detect the intestinal flora of feces. Results showed that chronic arsenic exposure could lead to neurobehavioral defects in rats, increase the expression levels of Tau, p‐Tau 396 , and Aβ 1–42 in hippocampus ( p < 0.05), increase the abundance of Clostridium _ UCG‐014, decrease the abundance of Roseburia , and decrease the expression levels of Claudin‐1 and Occludin in colon and hippocampus ( p < 0.05). After FMT intervention, the expression levels of Tau and p‐Tau 396 were decreased ( p < 0.05), and the abundance of Roseburia was increased. In summary, chronic arsenic exposure caused intestinal flora disorder by changing the a...