Hypercoagulable state and gut microbiota dysbiosis as predictors of poor functional outcomes in acute ischemic stroke patients

作者：Jinghong Li, Shengnan Chen, Siqi Yang, Wen Zhang, Xiaoqi Huang, Lang Zhou, Yanchao Liu, Mengxi Li, Yonghui Guo, Jia Yin, Kaiyu Xu · 发表于：mSystems · 年份：2025 · DOI：10.1128/msystems.01492-24 · 被引用次数：5 · 研究领域：Gut microbiota and health、Botulinum Toxin and Related Neurological Disorders、Meta-analysis and systematic reviews

ABSTRACT Stroke is the second leading cause of death worldwide. Acute ischemic stroke (AIS) patients often exhibit hypercoagulable state and gut microbiota dysbiosis. However, the association between coagulation abnormalities and gut microbiota dysbiosis in AIS patients and their predictive value for poor functional outcomes in AIS has not been investigated. Our study enrolled 95 AIS patients and 81 healthy controls, using 16S rRNA sequencing to analyze gut microbiota composition. Baseline fibrinogen level was found to be an independent risk factor for poor functional outcomes at 90-day follow-up (odds ratio = 2.16, 95% confidence interval: 1.02–4.59, P = 0.044). AIS patients showed significant gut microbiota dysbiosis, with significantly increased Parabacteroides and Alistipes , and decreased Prevotella and Roseburia , associated with coagulation indices. Furthermore, compared with AIS patients with normal coagulation function, those in a hypercoagulable state exhibited a significant increase in Alistipe s and a decrease in Prevotella . We identified gut microbial biomarkers consisting of 15 bacteria that predicted poor functional outcome in AIS patients at 90-day follow-up. Coagulation indices improved the predictive performance of these biomarkers. In training and validation cohorts, area under the curve (AUC) values were 0.930 and 0.890 for microbial biomarkers alone, 0.691 and 0.751 for coagulation indices alone, and 0.943 and 0.944 for coagulation indices combined with ...