Cry for help from rhizosphere microbiomes and self-rescue strategies cooperatively alleviate drought stress in spring wheat

作者：Jing Fang, Jie Ma, Tao Wen, Guoqing Niu, Shuli Wei, Shaofeng Su, Long Yi, Yuchen Cheng, Jun Yuan, Xiaoqing Zhao, Zhanyuan Lu · 发表于：Soil Biology and Biochemistry · 年份：2025 · DOI：10.1016/j.soilbio.2025.109813 · 被引用次数：43 · 研究领域：Plant-Microbe Interactions and Immunity、Agricultural Productivity and Crop Improvement、Soybean genetics and cultivation

In response to drought, plants modulate their morphology and orchestrate a range of functional adaptations. However, the intricate relationships between plants and their microbiome in response to drought stress are not fully understood. Herein, we used transcriptome and untargeted metabolomics technologies to study genetic and metabolic changes associated with drought resistance in spring wheat, as well as amplicon sequencing and metagenomic approaches were employed to investigate the influence of rhizosphere microorganisms on this process. Results indicated that plant functions of osmotic adjustment, oxidative stress, and stomatal regulation were enriched during drought conditions. Meanwhile, the relative abundances of trehalose, sucrose, gentiobiose, and abscisic acid in root exudates increased by 18.7 %, 21.1 %, 4.8 %, and 121.0 %, respectively. Cross-domain network construction with four omics data revealed that a significant increase in abundance of the trehalose biosynthetic pathway and sugar transporter SWEET gene promoted sucrose and trehalose secretion, respectively, leading to an enrichment of Pseudomonas and Streptomyces in the subsequent validation assay. Pseudomonas extremorientalis LS-8 and Streptomyces cinereoruber LW-5 were isolated to reveal that both strains could improve drought resistance by increasing the average aboveground fresh weight by more than 33.0 % and upregulating the expression of TaLEA2 , TaBADHb , TaWRKY10 , and TaERF3 in spring wheat. Taken ...