Fibrin induces infiltration of macrophages and neutrophils via integrin α M β 2 and triggers aortic dissection

作者：Jie Li, Jixiu Zhang, Lingwei Zou, Mingbo Cao, Zhonghui Zhu, Yu Li, Min Zhou, Weiguo Fu, Zhihui Dong, Huiyuan Gao, Baohong Jiang · 发表于：British Journal of Pharmacology · 年份：2025 · DOI：10.1111/bph.70033 · 被引用次数：4 · 研究领域：Aortic Disease and Treatment Approaches、Connective tissue disorders research、Aortic aneurysm repair treatments

Abstract Background and Purpose Infiltration of macrophages and neutrophils plays a crucial role in the occurrence of aortic dissection (AD), while the mechanism elucidating their infiltration remains unknown. The present study aimed to delineate the underlying mechanism and provide a potential therapeutic strategy to attenuate AD progression. Experimental Approach A model of AD was established in male mice using β ‐aminopropionitrile and angiotensin II. Proteomic analysis, histological evaluation, flow cytometry, western blot, multiple fluorescence staining and adhesion assays were used to evaluate fibrin and inflammatory cells during AD progression. Fibrinogen‐lowering drugs and fibrinogen γ ‐chain knockout (Fgg +/− ) mice were also used to evaluate the fibrin‐integrin α M β 2 interaction. Key Results Fibrin deposition was confirmed by proteomic analysis and histological staining, accompanied by infiltration of macrophages and neutrophils detected by flow cytometry during the progression of AD. After confirming that macrophages and neutrophils infiltrated at the sites where fibrin was deposited by immunofluorescence, an association between fibrin and the integrin α M β 2 was disclosed using protein–protein interaction analysis and immunofluorescence. The pivotal role of interactions between fibrin and integrin α M β 2 in AD progression was confirmed by cell adhesion in vitro, down‐regulation of fibrin using batroxobin and Fgg +/− mice in vivo. The relevance of fibrin and in...