Changes in the soil and rhizosphere microbiomes associated with bacterial wilt decline in the tomato monoculture field

作者：Junwei Peng, Jinfeng Hou, Hong Liu, Dmitri V. Mavrodi, Olga V. Mavrodi, Feifei Sun, Minchong Shen, Xia Wang, Keke Dang, Min Yan, Hui Liang, Yuanhua Dong, Jiangang Li · 发表于：Geoderma · 年份：2025 · DOI：10.1016/j.geoderma.2025.117273 · 被引用次数：5 · 研究领域：Plant-Microbe Interactions and Immunity、Nematode management and characterization studies、Composting and Vermicomposting Techniques

• Long-term tomato monoculture induces soil suppressiveness against Ralstonia wilt. • Soil suppressiveness links to increased Streptomyces and trace elements (Mn, Ni). • Suppressive soil leads to healthy rhizosphere community composition and function. • Bacillus and Pseudomonas are key rhizosphere microbes for suppressing wilt disease. Monoculture farming streamlines field equipment use and increases the efficiency of planting and harvesting, but at the same time, exacerbates the severity of soilborne diseases. Disease-suppressive soils are an effective and sustainable resource for managing soilborne diseases in monoculture systems. However, the evolution and mechanisms of soil suppressiveness remain elusive, limiting the broader acceptance of suppressive soil in agriculture. This study investigated changes in the belowground tomato microbiome during long-term monoculture leading to an outbreak and subsequent suppression of bacterial wilt, a destructive soilborne disease. The wilt incidence steadily increased, culminating in the most severe outbreak in the fifth cropping cycle. Surprisingly, in the seventh crop, wilt symptoms spontaneously declined, signifying a transition toward the disease-suppressive state. This transition involved the enrichment of Streptomyces and trace elements (Mn, Ni) in the bulk soil, accompanied by increased diversity and abundance of Pseudomonas in the rhizosphere . Greenhouse disease assays confirmed that the suppressive soil had significantly low...