Exploratory multimodal nonlinear microscopy: Coherent anti-stokes Raman scattering, second harmonic generation, and differential interference contrast for oral squamous cell carcinoma diagnosis

作者：Hangzhe Song, Xiaoxiao Li, T. Yang, Lifu Shao, Zhigang Sun, Penglong Cao, Rui Li, Juntao Ma · 发表于：Photodiagnosis and Photodynamic Therapy · 年份：2025 · DOI：10.1016/j.pdpdt.2025.104577 · 被引用次数：5 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Optical Coherence Tomography Applications、Photonic Crystal and Fiber Optics

OBJECTIVE: We applied multimodal nonlinear optical microscopy and Raman Spectroscopy to rapidly image Oral Squamous Cell Carcinoma (OSCC), comparing results with Hematoxylin-Eosin (H&E) stained to explore supplementary diagnostic methods. STUDY DESIGN: With ethical approval and patient informed consent, we processed primary tumor resection tissues, preparing frozen sections. Slice 1 was stained with H&E-stained, while Slice 2 was unstained and imaged using Coherent anti-Stokes Raman Scattering (CARS), Second-Harmonic Generation (SHG), Differential Interference Contrast (DIC), and Raman spectroscopy. These were analyzed alongside H&E-stained sections to assess diagnostic capabilities. RESULTS: . CARS imaging showed unique cancer nest structures, and SHG highlighted collagen fiber distribution. CONCLUSIONS: Multimodal nonlinear imaging, combining CARS, SHG, and DIC, shows potential as a supplementary diagnostic tool for oral squamous cell carcinoma, aiding in the characterization of pathological features and providing valuable clinical diagnostic information.