Co‐Expression Pattern Analysis of Head‐to‐Head NLR Gene Pair Pik‐H4

作者：Fengwei Gu, Huabin Xie, Qiwei Huang, Wenjie Zhou, Xiaodi Zou, Zhikai Han, Tao Guo, Hui Wang, Jiafeng Wang · 发表于：Plant Cell & Environment · 年份：2025 · DOI：10.1111/pce.15509 · 被引用次数：1 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Parasitism and Resistance、Toxin Mechanisms and Immunotoxins

ABSTRACT Nucleotide‐binding leucine‐rich repeat (NLR) genes play a critical role in plant effector‐triggered immunity (ETI) against pathogen invasion. However, the regulatory mechanisms governing NLR expression and functional dynamics, particularly in head‐to‐head NLR gene pairs, remain poorly understood. In this study, we investigated the regulatory mechanisms, subcellular localization and functional pathways associated with Pik‐H4 gene pair. Bidirectional Pik‐H4 promoter (P Pik‐H4 ) strengths were found across the whole plants and exhibited co‐expressed patterns in tissues and cells, and the P Pik‐H4 activity was upregulated in vascular bundles during blast fungus invasion. Additionally, altering the co‐expression of Pik 1 ‐H4 and Pik 2 ‐H4 via overexpression in rice or Nicotiana benthamiana did not compromise the immune response. Promoter analysis identified two minimal promoter regions that are essential for bidirectional transcription, and mutagenesis of the bidirectional TATA box confirmed its role in gene regulation. This dual‐function promoter coordinates Pik‐H4 expression in both directions, a regulatory innovation previously unreported in NLR‐mediated immunity. In planta subcellular localization revealed Pik 1 ‐H4 relocates to vesicles, indicating its role in effector recognition, while Pik 2 ‐H4 predominantly accumulated in the nucleus. These new discoveries of Pik protein extended the putative immune function of NLR pairs. Transcriptome analysis demonstrated that ...