Establishment and application of a qPCR method for differential detection of Brucella S2 vaccine strain

作者：Xianran Meng, Zheng Li, Yating Zhang, Yu Yu, WaGao, Wenlong Wang, Chunxia Liu · 发表于：BMC Veterinary Research · 年份：2025 · DOI：10.1186/s12917-025-04635-w · 被引用次数：7 · 研究领域：Brucella: diagnosis, epidemiology, treatment、Biosensors and Analytical Detection、Burkholderia infections and melioidosis

BACKGROUND: Brucellosis is one of the most serious zoonotic bacterial diseases in the world. The disease has caused serious harm to people and livestock, hindered the healthy development of the breeding industry, and led to serious economic losses.At present, the prevention and control of this kind of disease is still based on vaccine immunization. However, after the widely used vaccine is inoculated to livestock, there is no widely used differential diagnosis method to distinguish vaccine immune antibodies from natural infection antibodies. Quarantine and purification work is difficult to carry out. In addition, there are few studies using real-time PCR(qPCR) methods in the differential diagnosis of natural virulent strains and vaccine strains of brucellosis.The purpose of this study is to establish a rapid, sensitive and accurate differential diagnosis method for Brucella S2 vaccine strain, and to solve the problem of lack of identification of Brucella S2 vaccine strain and natural virulent strain in clinical detection.It avoids the killing of some livestock due to the positive antibody of the Brucella S2 vaccine strain, and can also identify sick animals from immune herds, reducing the economic losses of farms, and providing certain technical support for the quarantine and purification of epidemic diseases. RESULTS: copies/μL, the sensitivity is about 100 times higher than that of conventional PCR, and there is no cross-reaction with Escherichia coli,Salmonella,streptococc...