Increased temperature enhances microbial-mediated lignin decomposition in river sediment

作者：Jialing Li, Weimin Sun, Yingjie Cao, Jing Wu, Duan Li, Miaomiao Zhang, Xiaoqing Luo, Qi-Qi Deng, Ziqi Peng, Xiaozhen Mou, Wenjun Li, Pandeng Wang · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-025-02076-z · 被引用次数：14 · 研究领域：Enzyme-mediated dye degradation、Microbial Community Ecology and Physiology、Genomics and Phylogenetic Studies

Lignin, as the most abundant recalcitrant organic carbon in terrestrial ecosystems, plays a crucial role in the Earth’s carbon cycle. After lignin entering aquatic environments, portion of it tends to accumulate in sediments, forming a stable carbon relatively reservoir. However, the increasing temperature caused by human activities may impact microbial-mediated lignin decomposition, thereby affecting sedimentary carbon reservoirs. Therefore, revealing how temperature affects microbial-mediated lignin decomposition in river sediment, a topic that remains elusive, is essential for comprehending the feedbacks between river carbon reservoirs and climate. To address this, we conducted stable isotope probing of river surface sediment using 13C-lignin and 13C-vanillin, and utilized a series of techniques, including CO2 production analysis, 16S rRNA gene amplicon sequencing, metagenomics, and metatranscriptomics, to identify the lignin-decomposing microbes and the effects of temperature on microbial-mediated lignin decomposition. We found that elevated temperatures not only increased the total sediment respiration (total CO2) and the CO2 emissions from lignin/vanillin decomposition, but also enhanced priming effects. The 13C-labled taxa, including Burkholderiales, Sphingomonadales, and Pseudomonadales, were identified as the main potential lignin/vanillin decomposers, and their abundances and activity significantly increased as temperature increased. Furthermore, we observed that in...