Biohydrogen and Biobutanol Production from Spent Coffee and Tea Waste Using Clostridium beijerinckii

作者：Stephen A. Akinola, Beenish Saba, Ann D. Christy, Katrina Cornish, Thaddeus Chukwuemeka Ezeji · 发表于：Fermentation · 年份：2025 · DOI：10.3390/fermentation11040177 · 被引用次数：5 · 研究领域：Biofuel production and bioconversion、Microbial Metabolic Engineering and Bioproduction、Catalysis for Biomass Conversion

The growing advocacy for greener climates, coupled with increasing global energy demand driven by urbanization and population growth, highlights the need for sustainable solutions. Repurposing food wastes as substrates offers a promising approach to enhancing cleaner energy generation and promoting a circular economy. This study investigated the potential of spent coffee grounds (SC) and biosolids cake (BS) from tea wastes as substrates for producing valuable fuels and chemicals through acetone–ethanol–butanol (ABE) fermentation. Clostridium beijerinckii NCIMB 8052 was used to ferment 100% and 50% hydrolysates derived from Parr-treated enzyme-hydrolyzed (PEH, PEH50), Parr-treated non-hydrolyzed (PNEH, PNEH50), and non-Parr-treated hydrolyzed (NPEH) SC wastes, as well as enzyme-hydrolyzed (BSH, BSH50) and non-hydrolyzed BS wastes (NBH, NBH50). Fermentation of unmodified hydrolysates by C. beijerinckii was poor. Following CaCO3 modification of SC and BS hydrolysates, ABE titer, yield, and productivity increased, with the highest values obtained with PEH50 and NBH. Specifically, CaCO3 modification of SC hydrolysates led to increased butanol titer, yield, and productivity in PEH50, while the NBH exhibited higher butanol yield and productivity than the non-CaCO3-modified hydrolysates. Additionally, H2 gas production with PEH50 and NBH was 1.41- and 1.13-fold higher, respectively, than in other hydrolysates. These findings suggest that SC and BS hydrolysates can be valorized to but...