Biological functional analysis of common buckwheat (Fagopyrum esculentum) FeSGT1 gene in enhancing drought stress resistance

作者：Xudong Zhu, Lanfeng Yang, Yuanyuan Chen, Zehao Hou, Yi-Rou LUO, Ze-Hao XIONG, Zhengwu Fang · 发表于：ACTA AGRONOMICA SINICA · 年份：2023 · DOI：10.3724/sp.j.1006.2023.21028 · 研究领域：Seed and Plant Biochemistry、Magnetic and Electromagnetic Effects

SGT1 (Suppressor of the G2 Allele of skP1) is an inhibitor of skp1-4, which plays an important role in the abiotic stress response of plants. Based on the early transcriptomics and proteinomics analyses of common buckwheat under drought stress, a FeSGT1 gene was screened and cloned, which contained a 1086 bp open reading frame encoding 361 amino acids and 3 domains including TPR, CS, and SGS. Homologous protein comparison showed that FeSGT1 was closely related to CqSGT1 (XP_021726759.1), BvSGT1 (XP_010671588.1), and SoSGT1 (XP_021839743.1). Besides, FeSGT1 gene encoded membrane localization protein. The relative expression levels revealed that FeSGT1 tended to be up-regulated within 24 hours of drought stress. The expression of FeSGT1 gene peaked at 12 hours and began to decline after 24 hours under salt, low temperature (4℃), and ABA treatments. Overexpression of FeSGT1 gene in transgenic Arabidopsis not only conferred drought and salt tolerance, but also significantly increased root length, fresh weight, and survival rate compared with the wild type (WT) plant, accompanied by the elevated activities of catalase (CAT), the lowered malonaldehyde (MDA) and H2O2 contents, thus allowing plants to better adapt to adverse environments. Our results provided information in the exploring of the molecular regulation mechanism responding to drought tolerance in common buckwheat.