Gene mapping of apical spikelet degeneration mutant asd1 in wheat

作者：Qidi Du, Huijun Guo, Hongchun Xiong, Yongdun Xie, Linshu Zhao, Jiayu Gu, Shirong Zhao, Yuping Ding, Xiyun Song, Lu-Xiang LIU · 发表于：ACTA AGRONOMICA SINICA · 年份：2022 · DOI：10.3724/sp.j.1006.2022.11069 · 被引用次数：3 · 研究领域：Wheat and Barley Genetics and Pathology、Genetic Mapping and Diversity in Plants and Animals、Crop Yield and Soil Fertility

The number of grains per spike is one of the three key factors for yield and production in wheat. It is helpful to cultivate high-yield wheat varieties to elaborate the regulation pathway of spike development. A stable mutant of apical spikelet degeneration (asd1) was derived by EMS treatment, the wheat variety Jing 411 was used as wild type. The mutant asd1 showed that the apical spikelets were significantly degenerated, the spike length was shortened by 40%, the number of spikelets per spike was reduced by 35%, the number of grains per spike was significantly reduced by 54%, and the plant height was also significantly reduced. A genetic segregation population, Jing 411 × asd1, was developed, and phenotypic performances of both F2 and F3 were investigated in field. It showed that the degeneration of apical spikelet was controlled by a recessive gene. Using Bulked Segregant Analysis (BSA) and exon capture, SNPs were obtained and 7 KASP markers were further developed. The target mutation gene was mapped on chromosome 7A and narrowed down to a 9.91 Mb region on 7AS, which represented a genetic distance of 17.62 cM. The region had not yet been reported in regulating spikelet development and the target mutant gene might be a novel gene that controls the development of spike morphology in wheat, which will bring new insights for further understanding of the genetic basis of spikelet development in wheat.