Ganoderma lucidum Polysaccharide/carboxymethyl Chitosan Hydrogels Modulate Macrophage Polarization for Wound Healing

作者：Yu Zou, Yuheng Yang, Jingying Pei, Peilong Sun, Yan Wang · 发表于：Biomacromolecules · 年份：2025 · DOI：10.1021/acs.biomac.5c00112 · 被引用次数：26 · 研究领域：Wound Healing and Treatments、Polysaccharides and Plant Cell Walls、Fungal Biology and Applications

Wound healing remains a global challenge for clinical and experimental research. Hydrogels prepared from natural polysaccharides show great potential in the wound healing process. In this study, novel hydrogels (G-GLP) were prepared using oxidized Ganoderma lucidum polysaccharides (OGLPs) and carboxymethyl chitosan via the Schiff base reaction, which did not require the addition of any chemical cross-linking agent. The hydrogels showed excellent mechanical properties and biocompatibility. Moreover, the hydrogels showed superior hemostatic performance in mouse liver trauma and tail amputation models. Importantly, G-GLP improved inflammation by promoting the polarization of the macrophage M2 subtype, inhibiting the M1 subtype and reducing intracellular levels of reactive oxygen species. In vivo experiments demonstrated that G-GLP accelerated healing in a total defect wound model by reducing inflammation and promoting blood vessel repair and collagen deposition. These results demonstrate that G-GLP has potential as an effective wound repair dressing.