Foundry-fabricated dual-color nanophotonic neural probes for photostimulation and electrophysiological recording

作者：David A. Roszko, Fu‐Der Chen, John N. Straguzzi, Hannes Wahn, Alec Xu, Blaine McLaughlin, Xinxin Yin, Hongyao Chua, Xianshu Luo, Guo‐Qiang Lo, Joshua H. Siegle, Joyce K. S. Poon, Wesley D. Sacher · 发表于：Neurophotonics · 年份：2025 · DOI：10.1117/1.nph.12.2.025002 · 被引用次数：6 · 研究领域：Photoreceptor and optogenetics research、Neuroscience and Neural Engineering、Neural dynamics and brain function

Significance: Compact tools capable of delivering multicolor optogenetic stimulation to deep tissue targets with sufficient span, spatiotemporal resolution, and optical power remain challenging to realize. Here, we demonstrate foundry-fabricated nanophotonic neural probes for blue and red photostimulation and electrophysiological recording, which use a combination of spatial multiplexing and on-shank wavelength demultiplexing to increase the number of on-shank emitters. Aim: We demonstrate silicon (Si) photonic neural probes with 26 photonic channels and 26 recording sites, which were fabricated on 200-mm diameter wafers at a commercial Si photonics foundry. Each photonic channel consists of an on-shank demultiplexer and separate grating coupler emitters for blue and red light, for a total of 52 emitters. Approach: experiments by photostimulating through 16 of the available 26 emitter pairs. Results: We report neural probe electrode impedances, optical transmission, and beam profiles. We validated a packaged neural probe in optogenetic experiments with mice sensitive to blue or red photostimulation. Conclusions: Our foundry-fabricated nanophotonic neural probe demonstrates dense dual-color emitter integration on a single shank for targeted photostimulation. Given its two emission wavelengths, high emitter density, and long site span, this probe will facilitate experiments involving bidirectional circuit manipulations across both shallow and deep structures simultaneously.