Association between AHR in EGCs and IBS-D patients: the indole pathway of tryptophan metabolism

作者：Lianli Wang, Yue Zhang, Ran Yan, Laifu Li, Lin Mei, F Ye, Yating Sun, Ting Wang, Xiaojing Quan, Haitao Shi, Fei Dai · 发表于：Frontiers in Nutrition · 年份：2025 · DOI：10.3389/fnut.2025.1566595 · 被引用次数：6 · 研究领域：Gastrointestinal motility and disorders、Gut microbiota and health、Tryptophan and brain disorders

Background The pathophysiological mechanisms of irritable bowel syndrome (IBS) are intricate, and associated with tryptophan metabolites. This study was designed to investigate the relationship between indole metabolites in the feces and intestinal function in patients with IBS. Methods In this study, 42 patients with diarrhea-predominant IBS (IBS-D) and 36 healthy controls were recruited. The symptom severity was evaluated using IBS-quality of life (IBS-QOL) and IBS symptom severity system (IBS-SSS). The levels of indole metabolite in fecal samples were determined by means of mass spectrometry. Colon mucosal tissues were collected during colonoscopy procedures. Immunohistochemistry or immunofluorescence techniques were employed to analyze the expressions of the aryl hydrocarbon receptor (AHR), cytochrome P450 1A1 (CYP1A1), glial fibrillary acidic protein (GFAP), S100 calcium-binding protein B (S100B), zonula occludens-1 (Zo-1), occludin, substance P (SP), nerve growth factor (NGF), NOD-like receptor family pyrin domain containing 3 (NLRP3), and nuclear factor kappa B (NF-κB) in the mucosal tissues. Results Compared with healthy controls, the concentrations of the main indole metabolites ( p = 0.020), and the expressions of CYP1A1 ( p < 0.001), and Zo-1 ( p = 0.017) were decreased in patients with IBS-D, but the expressions of S100B ( p < 0.001), NF-κB ( p = 0.006), and NRLP3 ( p = 0.041) were increased. Immunofluorescence analysis demonstrated the co-expression...