A Nonconsumptive Fluorescent Probe for Precise Detection of Hydrogen Peroxide in Nonalcoholic Fatty Liver Disease and Inflammation

作者：Guixin Qin, Han‐Bo Zhang, Wei Shen, Yuting Wang, Nan Yin, Chenyao Nie, Juyoung Yoon, Qingling Xu · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.4c06647 · 被引用次数：17 · 研究领域：Sulfur Compounds in Biology、Molecular Sensors and Ion Detection、Eicosanoids and Hypertension Pharmacology

Hydrogen peroxide (H 2 O 2 ) plays a vital role in various physiological and pathological processes. Thus, fluorescent probes of H 2 O 2 are powerful tools for the investigation of H 2 O 2 -related diseases. However, developing fluorescent probes that do not irreversibly consume H 2 O 2 presents a significant challenge. In this work, we introduce carbonate ester as a nonconsumptive recognizing molecule to construct RES-6C as a novel fluorescent probe of H 2 O 2 . RES-6C exhibited a selective and sensitive turn-on fluorescence response to H 2 O 2, enabling the detection of H 2 O 2 in cells without disturbing the cellular redox status. RES-6C has been applied to study nonalcoholic fatty liver disease, revealing that peroxisomes and mitochondria contribute to H 2 O 2 production to a similar extent during very-long-chain fatty acid metabolism for the first time. It has also enabled fluorescent imaging of H 2 O 2 in the LPS-induced inflammation mouse model. Overall, RES-6C serves as a versatile tool to monitor H 2 O 2 in tissues and in vivo, providing new insights into the design of probes for H 2 O 2 .