Ultrasound-guided percutaneous radiofrequency ablation combined with anti-PD-1 for the treatment of prostate cancer: an experimental study

作者：Si Chen, Ruiqing Liu, Shaobo Duan, Beibei Zhang, Yuzhou Wang, Xiaoxiao Li, Yingying Zhao, Zesheng Li, Qi Zhou, Rui Zhang, Linlin Zhang, Linlin Zhang, Xiaoxia Xu, Ru Jang, Juan Zhang, Yaqiong Li, Xiguo Cai, Lianzhong Zhang, Lianzhong Zhang · 发表于：Frontiers in Oncology · 年份：2025 · DOI：10.3389/fonc.2025.1527763 · 被引用次数：2 · 研究领域：Ultrasound and Hyperthermia Applications、Hepatocellular Carcinoma Treatment and Prognosis、Cancer Immunotherapy and Biomarkers

Background: This study seeks to investigate the potential synergistic effects of combining ultrasound-guided percutaneous radiofrequency ablation with anti-PD-1 therapy on prostate cancer, utilizing animal models. Methods: , the mice were randomly divided into four groups and received corresponding intervention treatments. Among them, Group 1 was the blank control group, Group 2 was the simple anti-PD-1 treatment group, Group 3 was the simple radiofrequency ablation group, and Group 4 is the group that received percutaneous radiofrequency ablation combined with anti-PD-1 therapy under ultrasound guidance. The growth of the tumors was observed in mice after treatment in each group, tumor tissues were collected, and the immune status of the mice was analyzed through flow cytometry, immunohistochemistry, immunofluorescence, and other methods. Results: Compared with other treatment groups, ultrasound-guided percutaneous radiofrequency ablation combined with anti-PD-1 therapy significantly reduced the weight and volume of the tumors, demonstrating more effective tumor suppression. At the same time, combination therapy can promote the aggregation of T-cells within the tumor and increase the proportion of cytotoxic T-cells, increase the proportion of M1 macrophages and iNOS expression, and decrease the proportion of M2 macrophages and Arg expression in the local area of the tumors. Conclusion: Local ablation can improve the therapeutic effect of PD-1 monoclonal antibody. Our prelimi...