The effect of cyclic fluid perfusion on the proinflammatory tissue environment in osteoarthritis using equine joint-on-a-chip models

作者：Johannes Heidenberger, Eva I. Reihs, Jonathan Strauss, Martin Frauenlob, Sinan Gültekin, Iris Gerner, Stefan Tögel, Peter Ertl, Reinhard Windhager, Florien Jenner, Mario Rothbauer · 发表于：Lab on a Chip · 年份：2025 · DOI：10.1039/d4lc01078g · 被引用次数：9 · 研究领域：Osteoarthritis Treatment and Mechanisms、3D Printing in Biomedical Research、Microfluidic and Capillary Electrophoresis Applications

OA models face significant limitations in replicating human pathophysiology, particularly the complex interplay between joint tissues. Equine models, due to their anatomical and cellular similarities to humans, offer translational relevance but remain underutilized. This study aims to develop an advanced 3D coculture system using equine chondrocytes and synoviocytes to simulate tissue-level interactions and fluid mechanical forces involved in OA. By incorporating inflammatory stimuli and gravity-driven cyclic fluid actuation, this model enables the study of OA-related molecular interactions in both healthy and diseased conditions under dynamic fluid conditions. Findings from this research provide important insights into pathological tissue crosstalk. In turn, this can help to better understand underlying inflammatory pathways and the potential contribution of fluid flow as an influential factor on the tissue microenvironment.