DNA-Engineered Coating for Protecting the Catalytic Activity of Platinum Nanozymes in Biological Systems

作者：Lei Ren, Xia Liu, Shuai Tang, Yue Wang, Miao Yang, Linjie Guo, Jiang Li, Kai Jiao, Lihua Wang · 发表于：Biosensors · 年份：2025 · DOI：10.3390/bios15040205 · 被引用次数：4 · 研究领域：Advanced Nanomaterials in Catalysis、Advanced biosensing and bioanalysis techniques、Nanocluster Synthesis and Applications

Nanozymes, exemplified by metal nanoparticles, have shown promise in the fields of biological diagnostics and therapeutics. However, their practical application is often hindered by aggregation or deactivation in complex biological systems. Here, we develop a DNA-engineered nanozyme coating to preserve the peroxidase-like catalytic activity of platinum nanoparticles in complex biological environments. We employed thiol-modified single-stranded DNA to coat the platinum nanoparticles through metal-sulfur interaction. We found that the negatively charged DNA coating prevents the aggregation of platinum nanoparticles in high-salt environments. Moreover, the DNA coating functions as a molecular sieve, inhibiting non-specific protein adsorption while preserving substrate access to the catalytic interface, thus sustaining high peroxidase-like catalytic activity in serum. As a proof of concept, we demonstrate miRNA detection in serum samples with a detection limit of 1 fM. This approach offers a versatile strategy for molecular diagnostics of nanozymes in complex biological environments.