Sequential single-enzyme oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by an engineered lanthanide-dependent alcohol dehydrogenase

作者：Ke Liu, Ling Jiang, Lun Wang, Qunfeng Zhang, Lirong Yang, Jianping Wu, Haoran Yu · 发表于：Green Chemistry · 年份：2025 · DOI：10.1039/d5gc00157a · 被引用次数：12 · 研究领域：Catalysis for Biomass Conversion、Enzyme Catalysis and Immobilization、Biofuel production and bioconversion

A lanthanide-dependent alcohol dehydrogenase, PedH, was identified, which possessed the rare ability to continuously oxidize HMF to produce FDCA. A fully automated workflow was developed to modify PedH, resulting in an increased yield of FDCA.