Manure-Amended One-Year-Reclamation Promoted Soil Bacterial Phylotypic and Phenotypic Shifts in a Typical Coal-Mining Area

作者：Hongjuan Zhang, Yanmeng Shang, Shuning Bai, Meihua Fan, Xiaolong Sui, Huisheng Meng, Xianjun Hao, Xiangying Wang, Yulin Liu, Yi Li, Jianping Hong, Jie Zhang · 发表于：Microorganisms · 年份：2025 · DOI：10.3390/microorganisms13040699 · 被引用次数：3 · 研究领域：Microbial Community Ecology and Physiology、Soil Carbon and Nitrogen Dynamics、Mine drainage and remediation techniques

The initial variations in soil bacteria at the very beginning of reclamation still remains unclear. This study investigates the impact on bacterial communities of eight different treatments, including uncultivated land, unfertilized cultivation, chemical fertilizer, chemical fertilizer + bacterial fertilizer, manure, manure + bacterial fertilizer, manure + chemical fertilizer, and manure + chemical fertilizer + bacterial fertilizer, during the short-term reclamation of coal-mining soils. The results showed that total nitrogen, available phosphorus, soil organic carbon, microbial biomass carbon, and alkaline phosphatase activity were significantly increased in all fertilization treatments compared to uncultivated land (p < 0.05). All fertilization treatments other than chemical fertilizer harbored significantly higher activities of urease, catalase, and invertase than unfertilized cultivation (p < 0.05). The bacterial communities structures in manure-amended treatments significantly differed in uncultivated land and unfertilized cultivation and were phylotypically shifted from oligotrophic to Actinobacteria-dominant copiotrophic traits, accompanied with phenotypic succession of the enriching characteristics of Gram-positive, biofilms formation, and stress tolerance. The co-occurrence network in manure-amended treatments harbored a simple co-occurrence network, indicating more productive soils than in no-manure treatments. Manure amendment, total nitrogen, microbial bioma...