Combined transcriptomic and metabolomic analysis revealed the salt tolerance mechanism of Populus talassica × Populus euphratica

作者：Ying Liu, Mengxu Su, Xiaoqing Zhao, Meilin Liu, Jiaju Wu, Xiaofeng Wu, Zhanyuan Lu, Zhanjiang Han · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06288-1 · 被引用次数：21 · 研究领域：Plant Stress Responses and Tolerance、Plant Gene Expression Analysis、Plant Molecular Biology Research

BACKGROUND: To investigate the salt tolerance of Populus talassica × Populus euphratica, morphological and physiological parameters were measured on the second day after the 15th, 30th and 45th days of NaCl treatment, revealing significant effects of NaCl on growth. To further elucidate the mechanisms underlying salt tolerance, transcriptomic and metabolomic analysis were conducted under different NaCl treatments. RESULTS: The results of morphological and physiological indexes showed that under low salt treatment, P. talassica × P. euphratica was able to coordinate the growth of aboveground and belowground parts. Under high salt concentration, the growth and water balance of P. talassica × P. euphratica were markedly inhibited. The most significant differences between treatments were observed on the second day after the 45th day of NaCl treatment. Transcriptomic analysis showed that the pathways of gene enrichment in the roots and stems of P. talassica × P. euphratica were different in the salt resistance response. And it involves several core pathways such as plant hormone signal transduction, phenylpropanoid biosynthesis, MAPK signaling pathway-plant, plant- pathogen interaction, carbon metabolism, biosynthesis of amino acids, and several key Transcription factors (TFs) such as AP2/ERF, NAC, WRKY and bZIP. Metabolomic analysis revealed that KEGG pathway enrichment analysis showed unique metabolic pathways were enriched in P. talassica × P. euphratica under both 200 mM and 4...