De novo TANC2 variants caused developmental and epileptic encephalopathy and epilepsy

作者：Sheng Luo, Wenjun Zhang, Mi Jiang, Rong-Na Ren, Lei Liu, Yulan Li, Wenhui Liu, Pengyu Wang, Yujie Gu, Li‐Zhi Chen, Liping Shen, Yang Tian, Xiao‐Rong Liu, Yong‐Hong Yi, Wei‐Ping Liao, Peng Zhou, For the China Epilepsy Gene 1.0 Project · 发表于：Epilepsia · 年份：2025 · DOI：10.1111/epi.18358 · 被引用次数：18 · 研究领域：Epilepsy research and treatment、Single-cell and spatial transcriptomics、Neurogenesis and neuroplasticity mechanisms

OBJECTIVE: The TANC2 gene encodes a scaffolding synaptic protein with essential roles in synaptic transmission. This study aims to explore the association between TANC2 and epilepsy and the mechanism underlying phenotypic variation. METHODS: Trio-based exome sequencing was performed in patients with epilepsy from the China Epilepsy 1.0 cohort. The association between TANC2 and epilepsy was validated with a Drosophila model. The role of TANC2 in development was investigated by single-cell RNA sequencing in cerebral organoids and spatiotemporal expression across brain regions. RESULTS: De novo TANC2 variants were identified in six unrelated cases, including four null and two missense variants. The six variants were classified as "pathogenic"/"likely pathogenic," according to the American College of Medical Genetics and Genomics guidelines. Patients with null variants exhibited severe phenotypes, including three with epilepsy and neurodevelopmental disorders (NDDs) and one with developmental and epileptic encephalopathy (DEE). In contrast, the patients with missense variants presented with only epilepsy. Genotype-phenotype correlation analysis revealed that variants associated with epilepsy and NDD were mostly null variants, whereas the missense variants were associated with NDD or epilepsy. NDD-associated missense variants exhibited more severe damage effects, compared with the epilepsy-associated missense variants. Functional studies in Drosophila suggested that knockdown TANC...