[Effect of moxibustion combined with chemotherapy on immune checkpoints in tumor tissue of breast cancer-bearing mice].

作者：F. Zhang, Tianyu Gao, Chenxi Zhang, Ji-Juan Li, Xinyue Liang, Xiao‐Qi Zhang, Chun‐Sheng Jia, Lijia Pan · 发表于：PubMed · 年份：2025 · DOI：10.13702/j.1000-0607.20240144 · 被引用次数：3 · 研究领域：Medicine、Oncology、Cancer research、Internal medicine、Pathology、Immunology、Computer science、Artificial intelligence

OBJECTIVES: To observe the effects of moxibustion combined with chemotherapy on immune checkpoints including programmed cell death protein 1 (PD-1), T cell immunoglobulin domain and mucin domain (TIM-3) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) in the tumor tissue of breast cancer-bearing mice, so as to explore the effect and mechanisms of moxibustion combined with chemotherapy on breast cancer. METHODS: Forty BALB/c female mice were randomly divided into model, chemotherapy, moxibustion and combination groups, with 10 mice in each group. 4T1 tumor cells were inoculated into the fat pad under the left fourth nipple of the mice to establish the breast cancer-bearing mice model. The chemotherapy group was injected intraperitoneally with doxorubicin (DOX) solution at 2.5 mg/kg once every 3 days;the moxibustion group received bilateral "Zusanli" (ST36) moxibustion with 2 moxa cones per point, treated every 2 days;the combination group received both DOX injection and moxibustion intervention. The above interventions lasted for 21 days. The body weight and tumor volume of the mice were recorded daily. HE staining was used to observe tumor tissue pathological morphology. Immunohistochemistry and Western blot were used to detect the positive expression and protein expression levels of PD-1, TIM-3, and CTLA-4 in the tumor tissue. RESULTS: <0.01) of mice in the combination group. CONCLUSIONS: Moxibustion combined with chemotherapy shows more significant effects in inhibi...